ARG70080
Human IL36 beta recombinant protein (Active) (His-tagged, C-ter)
Human IL36 beta recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human IL36 beta recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IL36 beta |
| Species | Human |
| A.A. Sequence | Arg5 - Glu157 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is <0.2 ng/mL. |
| Alternate Names | FIL1; IL1-ETA; FILI-(ETA); IL-1F8; Interleukin-1 homolog 2; IL1H2; IL-1H2; FIL1-(ETA); Interleukin-1 eta; IL-1 eta; IL1F8; FIL1 eta; Interleukin-36 beta; Interleukin-1 family member 8; FIL1H |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL36B |
|---|---|
| Gene Full Name | interleukin 36, beta |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. Protein structure modeling indicated that this cytokine may contain a 12-stranded beta-trefoil structure that is conserved between IL1A (IL-A alpha) and IL1B (IL-1 beta). This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Stimulates production of interleukin-6 and interleukin-8 in synovial fibrobasts, articular chondrocytes and mature adipocytes. Induces expression of a number of antimicrobial peptides including beta-defensins 4 and 103 as well as a number of matrix metalloproteases. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of proinflammatory cytokines such as TNF-alpha, IL-8 and IL-6. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | N-terminal truncation leads to a dramatic enhancement of its activity (>1000-fold). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG70080 Human IL36 beta recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70080 Human IL36 beta recombinant protein (Active) (His-tagged, C-ter).
