ARG82610
Human IL35 ELISA Kit
Human IL35 ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82610 Human IL35 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL35 in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | This kit could assay both natural and recombinant Human IL35. No significant cross-reactivity or interference was observed in the following samples: Human: EBI3, gp130, IL1 beta, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL12 / IL35 p35, IL17A, IL18, IL21, IL22, IL23, IL27 and IL27RA. Mouse: EBI3, IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha. Rat: IFN gamma, IL1 beta, IL2, IL4, IL6, IL10, IL17A and TNF alpha. |
| Target Name | IL35 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.24 ng/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 0.469 ng/ml - 30 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: < 5% Inter-Assay CV: < 5% |
| Alternate Names | CLMF; Cytotoxic lymphocyte maturation factor 35 kDa subunit; NFSK; NK cell stimulatory factor chain 1; CLMF p35; NKSF1; IL-12 subunit p35; IL-12A; Interleukin-12 subunit alpha; P35; Interleukin 35; IL-35; IL35; Interleukin-27 subunit beta, EBI3; Human Interleukin 35 ELISA kit; Human IL-35 ELISA kit; |
Application Instructions
| Assay Time | ~ 4.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | IL12A + EBI3 |
| Gene Full Name | Interleukin 35 |
| Background | IL35 (Interleukin 35) is a dimeric protein composed of IL-12α and IL-27β chains, which are encoded by two separate genes called IL12A and EBI3, respectively. IL-35 receptor consists of IL-12Rβ2 (part of the IL-12R) and gp130 (part of IL-27R) chains. Compared to these two related interleukins, IL-35 is also able to signal through only one of the aforementioned chains. This was proven in vivo when absence of either of the receptor chains did not influence effects of IL-35. [provided by Wikipedia: Interleukin 35] |
| Function | IL35 (Interleukin 35) secreted by regulatory T-cells (Tregs), regulatory B-cells (Bregs) or even CD8+ regulatory T cells, IL-35 suppresses inflammatory responses of immune cells. IL-35 is not constitutively expressed in tissues, but the gene encoding IL-35 is transcribed by vascular endothelial cells, smooth muscle cells and monocytes after activation with proinflammatory stimuli. IL-35 has selective activities on different T-cell subsets; it induces proliferation of Treg cell populations but reduces activity of Th17 cell populations. [provided by Wikipedia: Interleukin 35] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82610 Human IL35 ELISA Kit standard curve image
ARG82610 Human IL35 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82610 Human IL35 ELISA Kit User manual |
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