ARG80151
Human IL21 ELISA Kit
Human IL21 ELISA Kit for ELISA and Human
Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80151-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80151-002 | Standard (Lyophilized) | 3 X 2.1 ng/vial | 4°C |
| ARG80151-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80151-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80151-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80151-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80151-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80151-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80151-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80151-010 | STOP solution | 12ml | 4°C |
| ARG80151-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80151 Human IL21 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL21 in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human IL-2,4,7,9,15；mouse IL-2,4,15,21 |
| Target Name | IL21 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 23.9 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 46.8 - 3000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | Za11; IL-21; CVID11; Interleukin-21 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL21 |
| Gene Full Name | interleukin 21 |
| Background | Interleukin-21 (IL-21) is a secreted, type-I cytokine with immunoregulatory activity. Human IL-21 shares the common ange of lymphoid and myeloid cells of the immune system (T cells, B cells, natural killer (NK) cells and dendritic cells). IL21 is therefore is able to regulate innate and acquired immune responses. The biological effects of IL-21 include induction of differentiation, maturation, and proliferation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Human IL-21 has also been shown to promote the anti-tumor activity of CD8+ T-cells and NK cells. During T-cell mediated immune response, IL21 may inhibit dendritic cells’ (DC) activation and maturation. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells; with the IL15, it may play a role in proliferation and maturation of natural killer (NK) cells. |
| Highlight | Related products: IL21 antibodies; IL21 ELISA Kits; IL21 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
Images (1) Click the Picture to Zoom In
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ARG80151 Human IL21 ELISA Kit standard curve image
ARG80151 Human IL21 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80151 Human IL21 ELISA Kit User's manual |
 Download
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