ARG80103

Human IL2 (High sensitive) ELISA Kit

Human IL2 (High sensitive) ELISA Kit for ELISA and Human

Immune System kit
publication_link Publication1

Component

Cat No Component Name Package Temp
ARG80103-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80103-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80103-003 Standard diluent buffer 20 ml 4°C
ARG80103-004 Antibody conjugate concentrate 400 µl 4°C
ARG80103-005 Antibody diluent buffer 16 ml 4°C
ARG80103-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80103-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80103-008 20X Wash buffer 50 ml 4°C
ARG80103-009 TMB substrate 12ml 4°C (Protect from light)
ARG80103-010 STOP solution 12ml 4°C
ARG80103-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80103 Human IL2 (High sensitive) ELISA Kit is a high sensitive Enzyme Immunoassay kit for the quantification of Human IL2 in Serum, Plasma, Cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human IL-2 R alpha, IL-2 R beta, IL-2 R gamma;mouse IL-2
Target Name IL2
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 1 pg/ml
Sample Type Serum, Plasma, Cell culture supernatants
Standard Range 1.95 - 125 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names TCGF; IL-2; lymphokine; Interleukin-2; Aldesleukin; T-cell growth factor

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3558 Human IL2

Swiss-port # P60568 Human Interleukin-2

Gene Symbol IL2
Gene Full Name interleukin 2
Background The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
Function Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. [UniProt]
Highlight Related products:
IL2 antibodies; IL2 ELISA Kits; IL2 recombinant proteins;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
Research Area Immune System kit

Images (1) Click the Picture to Zoom In

  • ARG80103 Human IL2 (High sensitive) ELISA Kit standard curve image

    ARG80103 Human IL2 (High sensitive) ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Engineering the chloroplast of Chlamydomonas reinhardtii to express the recombinant PfCelTOS-Il2 antigen-adjuvant fusion protein.

ELISA / Human

Shamriz S et al.
J Biotechnol.,  (2018)

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