ARG81544
Human IL18 binding protein ELISA Kit
Human IL18 binding protein ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81544-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81544-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81544-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81544-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81544-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81544-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81544-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81544-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81544-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81544-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81544-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81544 Human IL18 binding protein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL18 binding protein in serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | IL18 binding protein |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 46.85 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
| Standard Range | 93.7 - 6000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4% Inter-Assay CV: 6.7% |
| Alternate Names | IL18BPa; Tadekinig-alfa; IL-18BP; Interleukin-18-binding protein |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL18BP |
| Gene Full Name | interleukin 18 binding protein |
| Background | The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011] |
| Function | Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. [UniProt] |
| Highlight | Related products: IL18 binding protein antibodies; IL18 binding protein ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-glycosylated. O-glycosylated with core 1-like and core 2-like glycans. O-glycan heterogeneity at Ser-53: HexHexNAc (major) and Hex2HexNAc2 (minor). N-glycan heterogeneity at Asn-103: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); N-glycan at Asn-147: dHex1Hex5HexNAc4. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81544 Human IL18 binding protein ELISA Kit standard curve image
ARG81544 Human IL18 binding protein ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81544 Human IL18 binding protein ELISA Kit User's manual |
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