ARG82622
Human IL16 ELISA Kit
Human IL16 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82622-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82622-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82622-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82622-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82622-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82622-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82622-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82622-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82622-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82622-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82622-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82622 Human IL16 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL16 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | IL16 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3% Inter-Assay CV: 6.1% |
| Alternate Names | prIL-16; Lymphocyte chemoattractant factor; PRIL16; IL-16; NIL16; LCF; Pro-interleukin-16 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL16 |
| Gene Full Name | interleukin 16 |
| Background | The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010] |
| Function | Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane. Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. [UniProt] |
| Cellular Localization | Interleukin-16: Secreted. Isoform 1: Cytoplasm. Isoform 3: Cytoplasm. Nucleus. [UniProt] |
| Highlight | Related products: IL16 antibodies; IL16 ELISA Kits; IL16 Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82622 Human IL16 ELISA Kit standard curve image
ARG82622 Human IL16 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82622 Human IL16 ELISA Kit User manual |
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