ARG81624
Human IL15RA ELISA Kit
Human IL15RA ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81624-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81624-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81624-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81624-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81624-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81624-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81624-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81624-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81624-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81624-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81624-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81624 Human IL15RA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL15RA in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | IL15RA |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2% Inter-Assay CV: 7.4% |
| Alternate Names | sIL-15 receptor subunit alpha; sIL-15RA; Interleukin-15 receptor subunit alpha; IL-15R-alpha; IL-15RA; IL-15 receptor subunit alpha; CD antigen CD215; CD215; sIL-15R-alpha |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13261 Human Interleukin-15 receptor subunit alpha |
|---|---|
| Gene Symbol | IL15RA |
| Gene Full Name | interleukin 15 receptor, alpha |
| Background | This gene encodes a cytokine receptor that specifically binds interleukin 15 (IL15) with high affinity. The receptors of IL15 and IL2 share two subunits, IL2R beta and IL2R gamma. This forms the basis of many overlapping biological activities of IL15 and IL2. The protein encoded by this gene is structurally related to IL2R alpha, an additional IL2-specific alpha subunit necessary for high affinity IL2 binding. Unlike IL2RA, IL15RA is capable of binding IL15 with high affinity independent of other subunits, which suggests distinct roles between IL15 and IL2. This receptor is reported to enhance cell proliferation and expression of apoptosis inhibitor BCL2L1/BCL2-XL and BCL2. Multiple alternatively spliced transcript variants of this gene have been reported.[provided by RefSeq, Apr 2010] |
| Function | High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves SYK. [UniProt] |
| Highlight | Related products: IL15RA antibodies; IL15RA ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | A soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor. Phosphorylated by activated SYK. N-glycosylated and O-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81624 Human IL15RA ELISA Kit standard curve image
ARG81624 Human IL15RA ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81624 Human IL15RA ELISA Kit User's manual |
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