ARG80115
Human IL12 p70 ELISA Kit
Human IL12 p70 ELISA Kit for ELISA and Human
Immune System kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80115-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80115-002 | Standard (Lyophilized) | 3 X 4 ng/vial | 4°C |
| ARG80115-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80115-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80115-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80115-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80115-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80115-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80115-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80115-010 | STOP solution | 12ml | 4°C |
| ARG80115-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80115 Human IL12 p70 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL12 p70 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human IL-12 p35, IL-12/IL-23 p40, IL-23；mouse IL-12 p70 |
| Target Name | IL12 p70 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 8 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | IL12A; Interleukin 12A; IL-12A; NKSF1; CLMF; NFSK; P35; Interleukin 12A (Natural Killer Cell Stimulatory Factor 1, Cytotoxic Lymphocyte Maturation Factor 1, P35); Cytotoxic Lymphocyte Maturation Factor 35 KDa Subunit; Cytotoxic Lymphocyte Maturation Factor 1, P35; NF Cell Stimulatory Factor Chain 1; NK Cell Stimulatory Factor Chain 1; Interleukin-12 Subunit Alpha; Interleukin-12 Alpha Chain; Interleukin 12, P35; IL-12, Subunit P35; IL35 Subunit; CLMF P35; Natural Killer Cell Stimulatory Factor 1, 35 KD Subunit; IL-12 Subunit P35 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL12A |
| Gene Full Name | interleukin 12A |
| Background | This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. [UniProt] |
| Highlight | Related products: IL12 p70 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Immune System kit |
Images (1) Click the Picture to Zoom In
-
ARG80115 Human IL12 p70 ELISA Kit standard curve image
ARG80115 Human IL12 p70 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80115 Human IL12 p70 ELISA Kit User's manual |
 Download
|
