ARG81413
Human IL1 Receptor II ELISA Kit
Human IL1 Receptor II ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81413-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81413-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81413-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81413-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81413-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81413-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81413-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81413-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81413-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81413-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81413-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81413 Human IL1 Receptor II ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL1 Receptor II in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | IL1 Receptor II |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2% Inter-Assay CV: 7.0% |
| Alternate Names | IL-1 type II receptor; mIL-1RII; CDw121b; Interleukin-1 receptor type II; CD antigen CD121b; CD121b; Interleukin-1 receptor type 2; IL1R2c; sIL-1R2; IL1RB; Interleukin-1 receptor beta; sIL-1RII; IL-1R-2; IL-1RT2; IL-1RT-2; CD121 antigen-like family member B; mIL-1R2; IL-1R-beta |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL1R2 |
| Gene Full Name | interleukin 1 receptor, type II |
| Background | The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Alternative splicing results in multiple transcript variants and protein isoforms. Alternative splicing produces both membrane-bound and soluble proteins. A soluble protein is also produced by proteolytic cleavage. [provided by RefSeq, May 2012] |
| Function | Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. [UniProt] |
| Highlight | Related products: IL1 Receptor antibodies; IL1 Receptor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | A soluble form (sIL1R2) can also be produced by proteolytic cleavage at the cell surface (shedding) involving a metalloproteinase; hovever, several sIL1R2 forms ranging from 45 and 60 kDa are reported. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81413 Human IL1 Receptor II ELISA Kit standard curve image
ARG81413 Human IL1 Receptor II ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81413 Human IL1 Receptor II ELISA Kit User's manual |
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