ARG70259
Human IGFBP6 recombinant protein (His-tagged, C-ter)
Human IGFBP6 recombinant protein (His-tagged, C-ter) for Binding Activity,SDS-PAGE and Human
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) Human IGFBP6 recombinant protein. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | Binding, SDS-PAGE |
| Target Name | IGFBP6 |
| Species | Human |
| A.A. Sequence | Met1 - Gly240 of Human IGFBP6 (NP_002169.1) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
| Alternate Names | IBP-6; IGF-binding protein 6; IGFBP-6; IBP6; Insulin-like growth factor-binding protein 6 |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized recombinant human IGFBP6 at 1 ug/ml (100 µl/well) can bind recombinant human IGF1 with a linear range of 30-250 ng/ml. |
|---|
Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IGFBP6 |
|---|---|
| Gene Full Name | insulin-like growth factor binding protein 6 |
| Function | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 25 kDa |
| PTM | O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG70259 Human IGFBP6 recombinant protein (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70259 Human IGFBP6 recombinant protein (His-tagged, C-ter).
