ARG81411
Human IGFBP4 ELISA Kit
Human IGFBP4 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81411-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81411-002 | Standard | 2 X 32 ng/vial | 4°C |
| ARG81411-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81411-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81411-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81411-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81411-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81411-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81411-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81411-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81411-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81411 Human IGFBP4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IGFBP4 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | IGFBP4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 250 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 500 - 32000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 6.9% |
| Alternate Names | IBP4; IBP-4; IGFBP-4; Insulin-like growth factor-binding protein 4; HT29-IGFBP; IGF-binding protein 4; BP-4 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P22692 Human Insulin-like growth factor-binding protein 4 |
|---|---|
| Gene Symbol | IGFBP4 |
| Gene Full Name | insulin-like growth factor binding protein 4 |
| Background | This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008] |
| Function | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. [UniProt] |
| Highlight | Related products: IGFBP antibodies; IGFBP ELISA Kits; IGFBP Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81411 Human IGFBP4 ELISA Kit standard curve image
ARG81411 Human IGFBP4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81411 Human IGFBP4 ELISA Kit User's manual |
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