ARG82798
Human IGFBP1 ELISA Kit
Human IGFBP1 ELISA Kit for ELISA and Human
Overview
| Product Description | ARG82798 Human IGFBP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of IGFBP1 in Human serum. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Cross-Reactivity: Not react with IGFBP2, 3, 4 and 5. |
| Target Name | IGFBP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.5 µg/L |
| Sample Type | Serum |
| Standard Range | 1 - 250 µg/L |
| Sample Volume | 25 µl |
| Precision | Intra-Assay CV: 2.8% Inter-Assay CV: 6.2% |
| Alternate Names | IBP-1; IBP1; PP12; IGF-BP25; Insulin-like growth factor-binding protein 1; hIGFBP-1; IGFBP-1; Placental protein 12; AFBP; IGF-binding protein 1 |
Application Instructions
| Assay Time | 1 hour 15 min |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08833 Human Insulin-like growth factor-binding protein 1 |
|---|---|
| Gene Symbol | IGFBP1 |
| Gene Full Name | insulin-like growth factor binding protein 1 |
| Background | This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP N-terminal domain and a thyroglobulin type-I domain. The encoded protein, mainly expressed in the liver, circulates in the plasma and binds both insulin-like growth factors (IGFs) I and II, prolonging their half-lives and altering their interaction with cell surface receptors. This protein is important in cell migration and metabolism. Low levels of this protein may be associated with impaired glucose tolerance, vascular disease and hypertension in human patients. [provided by RefSeq, Aug 2017] |
| Function | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | Phosphorylated; probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82798 Human IGFBP1 ELISA Kit standard curve image
ARG82798 Human IGFBP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82798 Human IGFBP1 ELISA Kit User's manual |
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