ARG80157
Human IGFBP1 ELISA Kit
Human IGFBP1 ELISA Kit for ELISA and Human
Cell Biology and Cellular Response kit; Signaling Transduction kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80157-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80157-002 | Standard (Lyophilized) | 5 X 0.75 ng/vial | 4°C |
| ARG80157-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80157-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80157-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80157-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80157-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80157-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80157-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80157-010 | STOP solution | 12ml | 4°C |
| ARG80157-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80157 Human IGFBP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IGFBP1 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | IGFBP1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 25 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 46.8 - 3000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | IBP-1; IBP1; PP12; IGF-BP25; Insulin-like growth factor-binding protein 1; hIGFBP-1; IGFBP-1; Placental protein 12; AFBP; IGF-binding protein 1 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08833 Human Insulin-like growth factor-binding protein 1 |
|---|---|
| Gene Symbol | IGFBP1 |
| Gene Full Name | insulin-like growth factor binding protein 1 |
| Background | This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008] |
| Function | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. [UniProt] |
| Highlight | Related products: IGFBP1 antibodies; IGFBP1 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit; Signaling Transduction kit |
| PTM | Phosphorylated; probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis. |
Images (1) Click the Picture to Zoom In
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ARG80157 Human IGF-BP1 ELISA Kit standard curve image
ARG80157 Human IGF-BP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80157 Human IGFBP1 ELISA Kit User's manual |
 Download
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