ARG70385
Human IFN alpha recombinant protein (Active) (His-tagged, N-ter)
Human IFN alpha recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) Active Human IFN alpha recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | IFN alpha |
| Species | Human |
| A.A. Sequence | Cys24 - Glu189 |
| Expression System | E. coli |
| Protein Full Name | Interferon alpha-1/13 |
| Activity | Active |
| Activity Note | Determined by its ability to inhibit IL-8 secretion in human PBMCs in the presence of LPS. The ED50 for this effect is <1.12 μg/mL. |
| Alternate Names | IFNA1; Interferon Alpha 1; IFN-AlphaD; IFN-ALPHA IFNA13; IFNA@; IFL; IFN; Interferon Alpha-1/13; Interferon Alpha 1b; Interferon Alpha-D; IFN-Alpha-1/13; LeIF D; Interferon-Alpha1; IFN-Alpha 1b |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >95% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IFNA1 |
|---|---|
| Gene Full Name | Interferon Alpha 1 |
| Background | This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. This cytokine is upregulated in preeclamptic placentas and is thought to be a mediator of preeclampsia. |
| Function | Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. |
| Cellular Localization | Secreted |
| PTM | Disulfide bond |
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ARG70385 Human IFN alpha recombinant protein (Active) (His-tagged, N-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70385 Human IFN alpha recombinant protein (Active) (His-tagged, N-ter)
