ARG82698
Human IDS / Iduronate 2 Sulfatase ELISA Kit
Human IDS / Iduronate 2 Sulfatase ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82698-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82698-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82698-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82698-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82698-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82698-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82698-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82698-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82698-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82698-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82698-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82698 Human IDS / Iduronate 2 Sulfatase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IDS / Iduronate 2 Sulfatase in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | IDS / Iduronate 2 Sulfatase |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3% Inter-Assay CV: 7.6% |
| Alternate Names | Alpha-L-iduronate sulfate sulfatase; Iduronate 2-sulfatase; Idursulfase; SIDS; EC 3.1.6.13; MPS2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IDS |
| Gene Full Name | iduronate 2-sulfatase |
| Background | The protein encoded by this gene belongs to the sulfatase family, is localized to the lysosome, and is involved in lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease, mucopolysaccharidosis type II, also known as Hunter syndrome. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Aug 2013] |
| Function | Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. [UniProt] |
| Cellular Localization | Lysosome. [UniProt] |
| Highlight | Related products: Iduronate 2 Sulfatase antibodies; Iduronate 2 Sulfatase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82698 Human IDS / Iduronate 2 Sulfatase ELISA Kit standard curve image
ARG82698 Human IDS / Iduronate 2 Sulfatase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82698 Human IDS / Iduronate 2 Sulfatase ELISA kit User manual |
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