ARG83456
Human HtrA2 / Omi ELISA Kit
Human HtrA2 / Omi ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83456 Human HtrA2 / Omi ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human HtrA2 / Omi in Serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | HtrA2 / Omi |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | Serine protease HTRA2, mitochondrial; Serine proteinase OMI; Omi stress-regulated endoprotease; OMI; EC 3.4.21.108; PARK13; High temperature requirement protein A2; PRSS25; Serine protease 25; HtrA2 |
Application Instructions
| Assay Time | 3.5 hours |
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Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43464 Human Serine protease HTRA2, mitochondrial |
|---|---|
| Gene Symbol | HTRA2 |
| Gene Full Name | HtrA serine peptidase 2 |
| Background | This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq, Jul 2008] |
| Function | Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive. [UniProt] |
| Cellular Localization | Mitochondrion intermembrane space_x000D_Mitochondrion membrane; Single-pass membrane protein. [UniProt] |
| PTM | Autoproteolytically activated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83456 Human HtrA2 / Omi ELISA Kit standard curve image
ARG83456 Human HtrA2 / Omi ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83456 Human HtrA2 / Omi ELISA Kit User manual |
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