ARG81193
Human Hsc70 ELISA Kit
Human Hsc70 ELISA Kit for ELISA and Human
Overview
| Product Description | ARG81193 Human Hsc70 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Hsc70 in cell lysates, serum and tissue lysates. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Hsc 70 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 1.54 ng/ml |
| Sample Type | Cell lysates, serum and tissue lysates. |
| Standard Range | 2.34 - 150 ng/ml |
| Sample Volume | 100 µl |
| Alternate Names | NIP71; HEL-33; HSPA10; LAP-1; HSC70; HSC71; LAP1; Lipopolysaccharide-associated protein 1; Heat shock 70 kDa protein 8; HSC54; HEL-S-72p; Heat shock cognate 71 kDa protein; HSP71; HSP73; LPS-associated protein 1 |
Application Instructions
| Assay Time | 1.5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSPA8 |
| Gene Full Name | heat shock 70kDa protein 8 |
| Background | This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| Function | Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP. [UniProt] |
| Highlight | Related products: Hsc70 antibodies; Hsc70 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Acetylated. ISGylated. Trimethylation at Lys-561 reduces fibrillar SNCA binding. |
Images (1) Click the Picture to Zoom In
-
ARG81193 Human Hsc70 ELISA Kit standard curve image
ARG81193 Human Hsc70 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81193 Human Hsc70 ELISA Kit User manual |
 Download
|
