ARG83454
Human Hepcidin ELISA Kit
Human Hepcidin ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83454 Human Hepcidin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Hepcidin in Serum, plasma and cell culture supernatants., Urine |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Hepcidin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 4 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants., Urine |
| Standard Range | 7.8 - 500 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | HAMP; Hepcidin Antimicrobial Peptide; LEAP1; HEPC; LEAP-1; HFE2B; Liver-Expressed Antimicrobial Peptide 1; Putative Liver Tumor Regressor; Hepcidin; PLTR; Hepcidin Preproprotein |
Application Instructions
| Assay Time | 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HAMP |
| Gene Full Name | Hepcidin Antimicrobial Peptide |
| Background | he product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity against bacteria and fungi. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Oct 2014] |
| Function | Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. |
| Cellular Localization | Secreted |
| PTM | Cleavage on pair of basic residues, Disulfide bond |
Images (1) Click the Picture to Zoom In
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ARG83454 Human Hepcidin ELISA Kit standard curve image
ARG83454 Human Hepcidin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83454 Human Hepcidin ELISA Kit User manual |
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