ARG81618
Human HYAL1 ELISA Kit
Human HYAL1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81618-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81618-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81618-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81618-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81618-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81618-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81618-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81618-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81618-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81618-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81618-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81618 Human HYAL1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human HYAL1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | HYAL1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1% Inter-Assay CV: 6.8% |
| Alternate Names | LUCA1; Lung carcinoma protein 1; EC 3.2.1.35; NAT6; Hyal-1; LuCa-1; HYAL-1; Hyaluronoglucosaminidase-1; Hyaluronidase-1; MPS9 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HYAL1 |
| Gene Full Name | hyaluronoglucosaminidase 1 |
| Background | This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth. [UniProt] |
| Highlight | Related products: HYAL1 antibodies; HYAL1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81618 Human HYAL1 ELISA Kit standard curve image
ARG81618 Human HYAL1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81618 Human HYAL1 ELISA Kit User's manual |
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