ARG81581
Human HPX / Hemopexin ELISA Kit
Human HPX / Hemopexin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81581-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81581-002 | Standard | 2 X 200 ng/vial | 4°C |
| ARG81581-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81581-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81581-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81581-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81581-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81581-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81581-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81581-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81581-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81581 Human HPX / Hemopexin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human HPX / Hemopexin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | HPX / Hemopexin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 1.56 ng/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 3.12 - 200 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5% Inter-Assay CV: 6.5% |
| Alternate Names | Hemopexin; HX; Beta-1B-glycoprotein |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HPX |
| Gene Full Name | hemopexin |
| Background | This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq, Apr 2009] |
| Function | Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. [UniProt] |
| Highlight | Related products: HPX / Hemopexin antibodies; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylation in the 30-40 region is minor compared to glycosylation at Thr-24 and Thr-29. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81581 Human HPX / Hemopexin ELISA Kit standard curve image
ARG81581 Human HPX / Hemopexin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81581 Human Hemopexin ELISA Kit User's manual |
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