ARG83299
Human HNMT ELISA Kit
Human HNMT ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83299 Human HNMT ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human HNMT in Serum, Plasma and Cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | HNMT |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15 pg/ml |
| Detection Range | 62.5 pg/ml - 4,000 pg/ml |
| Sample Type | Serum, Plasma and Cell culture supernatants |
| Precision | Intra-Assay CV: 4.9% Inter-Assay CV: 4.7% |
| Alternate Names | HMT; Histamine N-methyltransferase; EC 2.1.1.8; HNMT-S1; HNMT-S2 |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HNMT |
| Gene Full Name | histamine N-methyltransferase |
| Background | In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine. [UniProt] |
| Cellular Localization | Cytoplasm. |
Images (1) Click the Picture to Zoom In
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ARG83299 Human HNMT ELISA Kit standard curve image
ARG83299 Human HNMT ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83299 Human HNMT ELISA Kit User manual |
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