ARG83024
Human HB EGF ELISA Kit
Human HB EGF ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83024 Human HB EGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human HB EGF in serum, plasma (heparin), ascites, urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Not cross-reacts with: Human Amphiregulin, Betacellulin, EGF, EGF R, Epigen, Epiregulin, NRG1F alpha, NRG1 beta1, TGF alpha. |
| Target Name | HB EGF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Protein Full Name | Proheparin-binding EGF-like growth factor |
| Sensitivity | 3.9 pg/ml |
| Sample Type | Serum, plasma (heparin), ascites, urine and cell culture supernatants. |
| Standard Range | 7.8 - 500 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | HBEGF, Heparin Binding EGF Like Growth Factor, HEGFL, DTR, DTS, Diphtheria Toxin Receptor (Heparin-Binding Epidermal Growth Factor Like Growth Factor), Diphtheria Toxin Receptor (Heparin Binding EGF-Like Growth Factor), Proheparin Binding EGF Like Growth Factor, Heparin Binding Epidermal Growth Factor, Heparin-Binding EGF-Like Growth Factor, DTSF |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99075 Human Proheparin-binding EGF-like growth factor |
|---|---|
| Gene Symbol | HBEGF |
| Gene Full Name | Heparin Binding EGF Like Growth Factor |
| Background | Enables growth factor activity and heparin binding activity. Involved in several processes, including epidermal growth factor receptor signaling pathway; positive regulation of protein kinase B signaling; and positive regulation of wound healing. Located in cell surface and extracellular space. Implicated in glomerulosclerosis and perinatal necrotizing enterocolitis. [provided by Alliance of Genome Resources, Apr 2022] |
| Function | Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. [UniProt] |
| Cellular Localization | Cell membrane, Membrane, Secreted. [UniProt] |
| PTM | Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83024 Human HB EGF ELISA Kit standard curve image
ARG83024 Human HB EGF ELISA Kit results of standard run with optical density reading at 450 nm
| Title | Download Link |
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| ARG83024 Human HB EGF ELISA Kit User manual |
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