ARG81582
Human Growth hormone receptor ELISA Kit
Human Growth hormone receptor ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81582-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81582-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81582-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81582-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81582-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81582-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81582-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81582-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81582-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81582-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81582-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81582 Human Growth hormone receptor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Growth hormone receptor in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Growth hormone Receptor |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3% Inter-Assay CV: 5.9% |
| Alternate Names | GH-binding protein; Growth hormone receptor; GHBP; GHIP; Serum-binding protein; GH receptor; Somatotropin receptor |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GHR |
| Gene Full Name | growth hormone receptor |
| Background | This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011] |
| Function | Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway (By similarity). The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. [UniProt] |
| Highlight | Related products: Growth hormone Receptor antibodies; Growth hormone Receptor ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. Shedding is inhibited by growth hormone (GH) binding to the receptor probably due to a conformational change in GHR rendering the receptor inaccessible to ADAM17 (By similarity). On GH binding, phosphorylated on tyrosine residues in the cytoplasmic domain by JAK2. On ligand binding, ubiquitinated on lysine residues in the cytoplasmic domain. This ubiquitination is not sufficient for GHR internalization (By similarity). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81582 Human Growth hormone receptor ELISA Kit standard curve image
ARG81582 Human Growth hormone receptor ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81582 Human Growth hormone receptor ELISA Kit User's manual |
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