ARG81543
Human Granulysin ELISA Kit
Human Granulysin ELISA Kit for ELISA and Human
Publication1
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81543-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81543-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81543-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81543-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81543-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81543-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81543-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81543-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81543-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81543-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81543-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81543 Human Granulysin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Granulysin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Granulysin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3% Inter-Assay CV: 6.6% |
| Alternate Names | Lymphokine LAG-2; LAG-2; Granulysin; LAG2; Protein NKG5; T-cell activation protein 519; TLA519; D2S69E; NKG5 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GNLY |
| Gene Full Name | granulysin |
| Background | The product of this gene is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. This protein is present in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Function | Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | A 9 kDa form is produced by proteolytic processing of a 15 kDa protein. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81543 Human Granulysin ELISA Kit standard curve image
ARG81543 Human Granulysin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
Specific References
Granulysin-mediated reduction of PDZRN3 induces Cx43 gap junctions activity exacerbating skin damage in trichloroethylene hypersensitivity syndrome
ELISA / Cell culture supernatants / Human
| Title | Download Link |
|---|---|
| ARG81543 Human Granulysin ELISA Kit User's manual |
 Download
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