ARG82383
Human Glypican 5 ELISA Kit
Human Glypican 5 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82383-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82383-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82383-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82383-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82383-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82383-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82383-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82383-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82383-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82383-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82383-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82383 Human Glypican 5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Glypican 5 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Glypican 5 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.9% Inter-Assay CV: 7.0% |
| Alternate Names | Glypican-5 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GPC5 |
| Gene Full Name | glypican 5 |
| Background | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008] |
| Function | Cell surface proteoglycan that bears heparan sulfate. [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Secreted glypican-5: Secreted, extracellular space. [UniProt] |
| Highlight | Related products: Glypican antibodies; Glypican ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82383 Human Glypican 5 ELISA Kit standard curve image
ARG82383 Human Glypican 5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82383 Human Glypican 5 ELISA Kit User manual |
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