ARG81861
Human GPNMB ELISA Kit
Human GPNMB ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81861-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81861-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81861-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81861-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81861-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81861-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81861-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81861-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81861-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81861-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81861-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81861 Human GPNMB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human GPNMB in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | GPNMB |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1%; Inter-Assay CV: 7.2% |
| Alternate Names | Transmembrane glycoprotein HGFIN; HGFIN; NMB; Transmembrane glycoprotein NMB |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GPNMB |
| Gene Full Name | glycoprotein (transmembrane) nmb |
| Background | The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Could be a melanogenic enzyme. [UniProt] |
| Highlight | Related products: GPNMB antibodies; GPNMB ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81861 Human GPNMB ELISA Kit standard curve image
ARG81861 Human GPNMB ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81861 Human GPNMB ELISA Kit User manual |
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