ARG82069
Human GM-CSF ELISA Kit
Human GM-CSF ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82069-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82069-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82069-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82069-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82069-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82069-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82069-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82069-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82069-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82069-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82069-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | Human GM-CSF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human GM-CSF in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | GM-CSF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.2%; Inter-Assay CV: 7.4% |
| Alternate Names | GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF; GMCSF; Molgramostin; Colony-stimulating factor; Sargramostim |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P04141 Human Granulocyte-macrophage colony-stimulating factor |
|---|---|
| Gene Symbol | CSF2 |
| Gene Full Name | colony stimulating factor 2 (granulocyte-macrophage) |
| Background | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: GM-CSF antibodies; GM-CSF ELISA Kits; GM-CSF Duos / Panels; GM-CSF recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82069 Human GM-CSF ELISA Kit standard curve image
ARG82069 Human GM-CSF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82069 Human GM-CSF ELISA Kit User's manual |
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