ARG83291
Human GLB1 / beta Galactosidase ELISA Kit
Human GLB1 / beta Galactosidase ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83291 Human GLB1 / beta Galactosidase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human GLB1 / beta Galactosidase in Serum, Plasma and Cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | GLB1 / beta Galactosidase |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15 pg/ml |
| Detection Range | 93.7 pg/ml - 6,000 pg/ml |
| Sample Type | Serum, Plasma and Cell culture supernatants |
| Precision | Intra-Assay CV: 5.5% Inter-Assay CV: 5.6% |
| Alternate Names | ELNR1; Lactase; MPS4B; EC 3.2.1.23; Elastin receptor 1; EBP; Acid beta-galactosidase; Beta-galactosidase |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GLB1 |
| Gene Full Name | galactosidase, beta 1 |
| Background | This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the terminal beta-galactose from ganglioside substrates and other glycoconjugates. Defects in this gene are the cause of GM1-gangliosidosis and Morquio B syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] |
| Function | Isoform 1: Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Isoform 2 has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers. [UniProt] |
| Cellular Localization | Cytoplasm; Lysosome |
| PTM | Disulfide bond; Glycoprotein; Zymogen |
Images (1) Click the Picture to Zoom In
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ARG83291 Human GLB1 / beta Galactosidase ELISA Kit standard curve image
ARG83291 Human GLB1 / beta Galactosidase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83291 Human GLB1 / beta Galactosidase ELISA Kit User manual |
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