ARG82532
Human GIF / Gastric intrinsic factor ELISA Kit
Human GIF / Gastric intrinsic factor ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82532-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82532-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82532-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82532-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82532-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82532-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82532-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82532-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82532-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82532-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82532-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82532 Human GIF / Gastric intrinsic factor ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human GIF / Gastric intrinsic factor in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | GIF / Gastric intrinsic factor |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 10 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.3% Inter-Assay CV: 5.5% |
| Alternate Names | Gastric intrinsic factor; IFMH; TCN3; Intrinsic factor; INF; IF |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GIF |
| Gene Full Name | gastric intrinsic factor (vitamin B synthesis) |
| Background | This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq, Jul 2008] |
| Function | Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: GIF antibodies; GIF ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82532 Human GIF / Gastric intrinsic factor ELISA Kit standard curve image
ARG82532 Human GIF / Gastric intrinsic factor ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82532 Human GIF / Gastric intrinsic factor ELISA Kit User manual |
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