ARG83038
Human GDNF ELISA Kit
Human GDNF ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83038 Human GDNF ELISA Kit is an Enzyme Immunoassay kit for the quantification ofHuman GDNF in serum, plasma (heparin), ascites, urine and cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | Cross-reacts with: Mouse GDNF (50%). Rat GDNF (50%). Not cross-reacts with: Human BDNF, CNTF, beta NGF, Syndecan 3, Neurturin. Mouse BDNF, CNTF, beta NGF, Neurturin. Rat BDNF, CNTF, beta NGF, Neurturin. |
| Target Name | GDNF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Protein Full Name | Glial cell line-derived neurotrophic factor |
| Sensitivity | 0.14 ng/ml |
| Sample Type | Serum, plasma (heparin), ascites, urine and cell culture supernatants. |
| Standard Range | 0.313 - 20 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | GDNF, Glial Cell Derived Neurotrophic Factor, Astrocyte-Derived Trophic Factor, HFB1 GDNF, ATF1, ATF2, Glial Cell Line Derived Neurotrophic Factor, ATF, Glial Cell Line Derived Neurotrophic Factor, Glial Derived Neurotrophic Factor, HSCR, HGDNF |
Application Instructions
| Assay Time | ~ 4 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P39905 Human Glial cell line-derived neurotrophic factor |
|---|---|
| Gene Symbol | GDNF |
| Gene Full Name | Glial Cell Derived Neurotrophic Factor |
| Background | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in this gene may be associated with Hirschsprung disease and Tourette syndrome. This gene encodes multiple protein isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016] |
| Function | Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. [UniProt] |
| Cellular Localization | Secreted |
| Highlight | Related products: GDNF antibodies; GDNF ELISA Kits; GDNF recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Cleavage on pair of basic residues, Disulfide bond, Glycoprotein |
Images (1) Click the Picture to Zoom In
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ARG83038 Human GDNF ELISA Kit standard curve image
ARG83038 Human GDNF ELISA Kit results of standard run with optical density reading at 450 nm
| Title | Download Link |
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| ARG83038 Human GDNF ELISA Kit User manual |
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