ARG70097
Human GDF11 recombinant protein (Active) (His-tagged, C-ter)
Human GDF11 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human GDF11 recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | GDF11 |
| Species | Human |
| A.A. Sequence | Asn299 - Ser407 |
| Expression System | E. coli |
| Activity | Active |
| Activity Note | Determined by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is <11 ng/mL. Determined by its ability to induce hemoglobin expression in K562 cells. The ED50 for this effect is <4 ng/mL. |
| Alternate Names | GDF-11; Bone morphogenetic protein 11; BMP11; Growth/differentiation factor 11; BMP-11 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >98% (by SDS-PAGE) |
| Buffer | 20 mM sodium citrate and 0.2 M NaCl (pH 3.5) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | GDF11 |
|---|---|
| Gene Full Name | growth differentiation factor 11 |
| Background | The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in mice and Xenopus suggest that this protein is involved in mesodermal formation and neurogenesis during embryonic development. [provided by RefSeq, Jul 2008] |
| Function | Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG70097 Human GDF11 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70097 Human GDF11 recombinant protein (Active) (His-tagged, C-ter).
