ARG83288
Human GCNT1 ELISA Kit
Human GCNT1 ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83288 Human GCNT1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human GCNT1 in Serum, Plasma and Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | GCNT1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15 pg/ml |
| Detection Range | 62.5 pg/ml - 4,000 pg/ml |
| Sample Type | Serum, Plasma and Cell culture supernatants |
| Precision | Intra-Assay CV: 4.4% Inter-Assay CV: 5.6% |
| Alternate Names | NAGCT2; Core 2 GNT; G6NT; C2GNT1; Core 2-branching enzyme; Core2-GlcNAc-transferase; NACGT2; C2GNT; EC 2.4.1.102; C2GNT-L; Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q02742 Human Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminylt |
|---|---|
| Gene Symbol | GCNT1 |
| Gene Full Name | glucosaminyl (N-acetyl) transferase 1, core 2 |
| Background | This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| Function | Glycosyltransferase that catalyzes the transfer of an N-acetylglucosamine moiety onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan. Mucin-type core 2 O-glycans play an important role in leukocyte extravasation as they serve as scaffolds for the display of the selectin ligand sialyl Lewis X by leukocytes. [UniProt] |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein. Note=Also detected in the trans-Golgi network. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83288 Human GCNT1 ELISA Kit standard curve image
ARG83288 Human GCNT1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83288 Human GCNT1 ELISA Kit User manual |
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