ARG81480
Human Furin ELISA Kit
Human Furin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81480-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81480-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81480-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81480-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81480-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81480-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81480-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81480-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81480-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81480-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81480-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81480 Human Furin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Furin in cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Furin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3% Inter-Assay CV: 5.8% |
| Alternate Names | FUR; Paired basic amino acid residue-cleaving enzyme; PCSK3; EC 3.4.21.75; PACE; Dibasic-processing enzyme; SPC1; Furin |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FURIN |
| Gene Full Name | furin (paired basic amino acid cleaving enzyme) |
| Background | This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140 and may play a role in tumor progression. This gene is located in close proximity to family member proprotein convertase subtilisin/kexin type 6 and upstream of the FES oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. [UniProt] |
| Highlight | Related products: Furin antibodies; Furin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation. Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81480 Human Furin ELISA Kit standard curve image
ARG81480 Human Furin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81480 Human Furin ELISA Kit User's manual |
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