ARG81590

Human Fetuin B ELISA Kit

Human Fetuin B ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG81590-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81590-002 Standard 2 X 10 ng/vial 4°C
ARG81590-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81590-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81590-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81590-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81590-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81590-008 25X Wash buffer 20 ml 4°C
ARG81590-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81590-010 STOP solution 10 ml (Ready to use) 4°C
ARG81590-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81590 Human Fetuin B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Fetuin B in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name Fetuin B
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.7%
Inter-Assay CV: 6.3%
Alternate Names IRL685; Fetuin-like protein IRL685; Fetuin-B; Gugu; 16G2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 26998 Human FETUB

Swiss-port # Q9UGM5 Human Fetuin-B

Gene Symbol FETUB
Gene Full Name fetuin B
Background The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells. [provided by RefSeq, Jul 2008]
Function Protease inhibitor required for egg fertilization. Required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity). [UniProt]
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  • ARG81590 Human Fetuin B ELISA Kit standard curve image

    ARG81590 Human Fetuin B ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81590 Human Fetuin B ELISA Kit User's manual download icon Download