ARG81134
Human Factor XIII ELISA Kit
Human Factor XIII ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81134-001 | Antibody-coated Microtiter Plate | 12 x 8 strips | 4°C |
| ARG81134-002 | 20X wash buffer concentrate | 30ml X 2 bottles | 4°C |
| ARG81134-003 | Plate sealer | 3 pieces | 4°C |
| ARG81134-004 | Biotinylated Human Factor XIII antibody (50X) | 1 vial (140 μl) | 4°C |
| ARG81134-005 | Human FXIII Standard | 1 vial (160ng, lyophilized) | 4°C |
| ARG81134-006 | 10X EIA Diluent | 30 ml | 4°C |
| ARG81134-007 | 100X Streptavidin-HRP | 80 μl | 4°C |
| ARG81134-008 | TMB Substrate | 8 ml | 4°C, ready for use |
| ARG81134-009 | STOP solution | 12 ml | 4°C, ready for use |
Overview
| Product Description | ARG81134 Human Factor XIII ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Factor XIII in serum, plasma, cell culture, urine, saliva, milk and CSF. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Factor XIII |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 2.1 ng/ml |
| Sample Type | Serum, plasma, cell culture, urine, saliva, milk and CSF |
| Standard Range | 2.5 - 160 ng/ml |
| Sample Volume | 50 µl |
| Precision | Intra-Assay CV: 4.4% Inter-Assay CV: 9.4% |
| Alternate Names | Coagulation factor XIIIa; F13A; Protein-glutamine gamma-glutamyltransferase A chain; Coagulation factor XIII A chain; Transglutaminase A chain; EC 2.3.2.13 |
Application Instructions
| Assay Time | ~ 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | F13A1 |
| Gene Full Name | coagulation factor XIII, A1 polypeptide |
| Background | This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008] |
| Function | Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. [UniProt] |
| Highlight | Related products: Factor XIII antibodies; Factor XIII ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The activation peptide is released by thrombin. |
Images (1) Click the Picture to Zoom In
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ARG81134 Human Factor XIII ELISA Kit standard curve image
ARG81134 Human Factor XIII ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81134 Human Factor XIII ELISA Kit User's manual |
 Download
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