ARG70509
Human Factor X recombinant protein (His-tagged)
Human Factor X recombinant protein (His-tagged) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged Human Factor X recombinant protein. |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Factor X |
| Species | Human |
| A.A. Sequence | Ala41-Arg182 |
| Expression System | CHO |
| Protein Full Name | Coagulation factor X |
| Alternate Names | F10; Coagulation Factor X; Stuart-Prower Factor; EC 3.4.21.6; Prothrombinase; Stuart Factor; Factor Xa; EC 3.4.21; Factor X; FXA; FX |
Properties
| Form | Powder |
|---|---|
| Purification | >90% (by SDS-PAGE) |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | F10 |
|---|---|
| Gene Full Name | Coagulation Factor X |
| Background | This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015] |
| Function | Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. [UniProt] |
