ARG83282
Human Factor X ELISA Kit
Human Factor X ELISA Kit for ELISA and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | ARG83282 Human Factor X ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Factor X in Serum, Plasma and Cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Factor X |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 25 pg/ml |
| Detection Range | 0.78 ng/ml - 50 ng/ml |
| Sample Type | Serum, Plasma and Cell culture supernatants |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 6.0% |
| Alternate Names | FX; Stuart factor; EC 3.4.21.6; Stuart-Prower factor; FXA; Coagulation factor X |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | F10 |
| Gene Full Name | coagulation factor X |
| Background | Factor X is the first enzyme in the common pathway of blood coagulation. It can be activated by Factor IXa (with Factor VIII cofactor) of the contact pathway and by Factor VIIa of the tissue factor pathway. After activation, It cleaves prothrombin to form thrombin. Thrombin not only transforms Fibrinogen to form Fibrin and but also activates Factor XIII to stabilize Fibrin in the thrombus. |
| Function | Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody |
| PTM | The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway). The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. |
Images (1) Click the Picture to Zoom In
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ARG83282 Human Factor X ELISA Kit standard curve image
ARG83282 Human Factor X ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83282 Human Factor X ELISA Kit User manual |
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