ARG70508
Human Factor IX recombinant protein (His-tagged)
Human Factor IX recombinant protein (His-tagged) for SDS-PAGE
Overview
| Product Description | CHO expressed, His-tagged Factor IX recombinant protein. |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Factor IX |
| Species | Human |
| A.A. Sequence | Met1-Thr461 |
| Expression System | CHO |
| Protein Full Name | Coagulation factor IX |
| Alternate Names | F9; Coagulation Factor IX; FIX; Plasma Thromboplastic Component; Plasma Thromboplastin Component; Christmas Factor ; EC 3.4.21.22; PTC; Christmas Disease; Hemophilia B; Factor IX F9; Factor IX; EC 3.4.21; Factor 9; F9 P22; THPH8; HEMB; P19 |
Properties
| Form | Powder |
|---|---|
| Purification | >95% (by SDS-PAGE) |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | F9 |
|---|---|
| Gene Full Name | Coagulation Factor IX |
| Background | This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Sep 2015] |
| Function | Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ ions, phospholipids, and factor VIIIa. [UniProt] |
