ARG82722
Human FUT8 ELISA Kit
Human FUT8 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82722-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82722-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82722-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82722-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82722-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82722-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82722-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82722-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82722-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82722-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82722-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82722 Human FUT8 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FUT8 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | FUT8 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 6.9% |
| Alternate Names | Alpha-(1,6)-fucosyltransferase; Alpha1-6FucT; EC 2.4.1.68; Fucosyltransferase 8; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; GDP-fucose--glycoprotein fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FUT8 |
| Gene Full Name | fucosyltransferase 8 (alpha (1,6) fucosyltransferase) |
| Background | This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2011] |
| Function | Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. [UniProt] |
| Cellular Localization | Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Tyrosine phosphorylated by PKDCC/VLK. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82722 Human FUT8 ELISA Kit standard curve image
ARG82722 Human FUT8 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82722 Human FUT8 ELISA kit User manual |
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