ARG81603
Human FOLR2 ELISA Kit
Human FOLR2 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81603-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81603-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81603-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81603-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81603-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81603-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81603-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81603-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81603-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81603-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81603-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81603 Human FOLR2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FOLR2 in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | FOLR2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0% Inter-Assay CV: 7.6% |
| Alternate Names | Folate receptor 2; BETA-HFR; FBP; Folate receptor beta; Folate receptor, fetal/placental; FBP/PL-1; FR-beta; FR-P3; Placental folate-binding protein; FR-BETA |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FOLR2 |
| Gene Full Name | folate receptor 2 (fetal) |
| Background | The protein encoded by this gene is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. [UniProt] |
| Highlight | Related products: FOLR2 antibodies; FOLR2 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81603 Human FOLR2 ELISA Kit standard curve image
ARG81603 Human FOLR2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81603 Human FOLR2 ELISA Kit User's manual |
 Download
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