ARG82448

Human FLRT3 ELISA Kit

Human FLRT3 ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82448-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82448-002 Standard 2 X 10 ng/vial 4°C
ARG82448-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82448-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82448-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82448-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82448-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82448-008 25X Wash buffer 20 ml 4°C
ARG82448-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82448-010 STOP solution 10 ml (Ready to use) 4°C
ARG82448-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82448 Human FLRT3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FLRT3 in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name FLRT3
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.1%
Inter-Assay CV: 5.7%
Alternate Names HH21; Leucine-rich repeat transmembrane protein FLRT3; Fibronectin-like domain-containing leucine-rich transmembrane protein 3

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23767 Human FLRT3

Swiss-port # Q9NZU0 Human Leucine-rich repeat transmembrane protein FLRT3

Gene Symbol FLRT3
Gene Full Name fibronectin leucine rich transmembrane protein 3
Background This gene encodes a member of the fibronectin leucine rich transmembrane protein (FLRT) family. FLRTs may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. This gene is expressed in many tissues. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2010]
Function May have a function in cell adhesion and/or receptor signaling. [UniProt]
Cellular Localization Cell membrane; Single-pass membrane protein. Endoplasmic reticulum membrane. Cell junction, focal adhesion. Secreted. Cell projection, axon. Note=Detected on dendritic punctae that colocalize in part with glutamaergic synapses, but not with GABAergic synapses. Detected on axons and at axon termini. Detected at neuronal growth cones. Proteolytic cleavage in the juxtamembrane region gives rise to a shedded ectodomain. [UniProt]
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PTM N-glycosylated.

Proteolytic cleavage in the juxtamembrane region gives rise to a soluble ectodomain. Cleavage is probably effected by a metalloprotease. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG82448 Human FLRT3 ELISA Kit standard curve image

    ARG82448 Human FLRT3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82448 Human FLRT3 ELISA Kit User manual download icon Download