ARG70412
Human FGF8 (isoform B) recombinant protein (Active) (His-tagged, C-ter)
Human FGF8 (isoform B) recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) Active Human FGF8 (isoform B) recombinant protein |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | FGF8 (isoform B) |
| Species | Human |
| A.A. Sequence | Gln52 - Arg223 |
| Expression System | E. coli |
| Protein Full Name | Fibroblast growth factor 8 |
| Activity | Active |
| Activity Note | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 1.4-3.8 ng/mL. The specific activity of recombinant human FGF-8b is > 2 x 10^5 IU/mg. |
| Alternate Names | FGF8; Fibroblast Growth Factor 8; AIGF; Androgen-Induced Growth Factor; Fibroblast Growth Factor 8 (Androgen-Induced); Heparin-Binding Growth Factor 8; HBGF-8; FGF-8; KAL6; HH6 |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | >95% (by SDS-PAGE) |
| Buffer | PBS (pH 8.0) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not <200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF8 |
|---|---|
| Gene Full Name | Fibroblast Growth Factor 8 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants. |
| Function | Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. |
| Cellular Localization | Secreted |
| PTM | Glycoprotein |
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ARG70412 Human FGF8 (isoform B) recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70412 Human FGF8 (isoform B) recombinant protein (Active) (His-tagged, C-ter).
