ARG80158
Human FGF21 ELISA Kit
Human FGF21 ELISA Kit for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80158-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80158-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80158-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80158-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80158-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80158-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80158-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80158-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80158-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80158-010 | STOP solution | 12ml | 4°C |
| ARG80158-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80158 Human FGF21 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FGF21 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human FGF-19, FGF-23, FGF R1 (IIIb)/Fc Chimera, FGF R1 (IIIc)/Fc Chimera, FGF R2 (IIIb)/Fc Chimera, FGF R2 (IIIc)/Fc Chimera, Klotho；mouse FGF R2 (IIIb)/Fc Chimera, FGF R2 (IIIc)/Fc Chimera |
| Target Name | FGF21 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 31.3 pg/ml |
| Sample Type | Serum, Plasma, Cell culture supernatants |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: less than10% |
| Alternate Names | Fibroblast growth factor 21; FGF-21 |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FGF21 |
| Gene Full Name | fibroblast growth factor 21 |
| Background | FGF-21 is a secreted growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-21, in the presence of betaKlotho as a protein cofactor, signals through the FGFR 1c and 4 receptors and stimulates insulin independent glucose uptake by adipocytes. Recombinant human FGF-21 is a 19.5 kDa protein containing 182 amino acid residues. |
| Highlight | Related products: FGF21 antibodies; FGF21 ELISA Kits; FGF21 recombinant proteins; Related news: A new role of fat – factory of circulating miRNAs New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit |
Images (1) Click the Picture to Zoom In
-
ARG80158 Human FGF21 ELISA Kit standard curve image
ARG80158 Human FGF21 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80158 Human FGF21 ELISA Kit User's manual |
 Download
|
