ARG81511
Human FGF19 ELISA Kit
Human FGF19 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81511-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81511-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81511-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81511-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81511-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81511-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81511-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81511-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81511-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81511-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81511-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81511 Human FGF19 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FGF19 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | FGF19 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9% Inter-Assay CV: 6.3% |
| Alternate Names | Fibroblast growth factor 19; FGF-19 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FGF19 |
| Gene Full Name | fibroblast growth factor 19 |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. [provided by RefSeq, Jul 2008] |
| Function | Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. [UniProt] |
| Highlight | Related products: FGF19 antibodies; FGF19 ELISA Kits; FGF19 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81511 Human FGF19 ELISA Kit standard curve image
ARG81511 Human FGF19 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81511 Human FGF19 ELISA Kit User's manual |
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