ARG81541
Human FCN3 ELISA Kit
Human FCN3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81541-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81541-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG81541-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81541-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81541-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81541-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81541-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81541-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81541-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81541-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81541-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81541 Human FCN3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FCN3 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | FCN3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.3% Inter-Assay CV: 7.0% |
| Alternate Names | Hakata antigen; Collagen/fibrinogen domain-containing protein 3; Collagen/fibrinogen domain-containing lectin 3 p35; Ficolin-3; HAKA1; FCNH |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FCN3 |
| Gene Full Name | ficolin (collagen/fibrinogen domain containing) 3 |
| Background | Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum, there are two types of ficolins, both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein, which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus, has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants, each encoding a distinct isoform, have been identified. [provided by RefSeq, Jul 2008] |
| Function | May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Has affinity with GalNAc, GlcNAc, D-fucose, as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota. [UniProt] |
| Highlight | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The N-terminus is blocked. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81541 Human FCN3 ELISA Kit standard curve image
ARG81541 Human FCN3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81541 Human FCN3 ELISA Kit User's manual |
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