ARG81539
Human FCN1 ELISA Kit
Human FCN1 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81539-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81539-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG81539-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81539-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81539-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81539-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81539-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81539-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81539-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81539-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81539-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81539 Human FCN1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FCN1 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | FCN1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9% Inter-Assay CV: 6.2% |
| Alternate Names | Ficolin-A; Collagen/fibrinogen domain-containing protein 1; Ficolin-alpha; Ficolin-1; M-ficolin; FCNM |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FCN1 |
| Gene Full Name | ficolin (collagen/fibrinogen domain containing) 1 |
| Background | The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct. Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity. [provided by RefSeq, Jul 2008] |
| Function | Extracellular lectin functioning as a pattern-recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8. Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. [UniProt] |
| Highlight | Related products: FCN1 antibodies; FCN1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81539 Human FCN1 ELISA Kit standard curve image
ARG81539 Human FCN1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81539 Human FCN1 ELISA Kit User's manual |
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