ARG82624
Human FABP2 / Intestinal FABP ELISA Kit
Human FABP2 / Intestinal FABP ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82624-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82624-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82624-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82624-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82624-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82624-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82624-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82624-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82624-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82624-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82624-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82624 Human FABP2 / Intestinal FABP ELISA KitPPPPP is an Enzyme Immunoassay kit for the quantification of Human FABP2 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | FABP2 / Intestinal FABP |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 31.3 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6% Inter-Assay CV: 7.2% |
| Alternate Names | Intestinal-type fatty acid-binding protein; I-FABP; Fatty acid-binding protein, intestinal; Fatty acid-binding protein 2; FABPI |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P12104 Human Fatty acid-binding protein, intestinal |
|---|---|
| Gene Symbol | FABP2 |
| Gene Full Name | fatty acid binding protein 2, intestinal |
| Background | The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq, Jul 2008] |
| Function | FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Highlight | Related products: FABP2 antibodies; FABP2 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82624 Human FABP2 / Intestinal FABP ELISA Kit standard curve image
ARG82624 Human FABP2 / Intestinal FABP ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82624 Human FABP2 / Intestinal FABP ELISA Kit User manual |
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