ARG80842
Human Estriol (unconjugated) ELISA Kit
Human Estriol (unconjugated) ELISA Kit for ELISA and Human
Cell Biology and Cellular Response kit; Signaling Transduction kit
Overview
| Product Description | ARG80842 Human Estriol (unconjugated) ELISA Kit is an enzyme immunoassay kit for the quantification of free estriol (unconjugated estriol) in serum during the second half of pregnancy. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Estriol |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 0.02 ng/ml |
| Sample Type | Serum. |
| Standard Range | 0.3 - 40 ng/ml |
| Sample Volume | 10 µl |
Application Instructions
| Assay Time | 1h, 30 min |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Estriol, unconjugated |
|---|---|
| Background | Estriol (E3) is the major estrogen formed by the fetoplacental unit during pregnancy. Unconjugated E3 passes through the placenta into the maternal circulation, where it is rapidly converted into glucuronide and sulfate derivatives to facilitate its excretion. The half-life of estriol in the maternal bloodstream is only 20-30 minutes. Its measurement, therefore offers a convenient and quick evaluation of current fetal status. Plasma estriol levels increase steadily throughout pregnancy and most rapidly during the third trimester (28-40 weeks). A sudden decrease in fetoplacental E3 production will result in a rapid fall in unconjugated E3 in the maternal serum. There are several potential advantages to measuring unconjugated E3 rather than total serum or urinary E3. Unconjugated estriol levels are free from effects related to maternal renal or hepatic disease, and are not altered by the administration of certain antibiotics. Unconjugated E3 more accurately reflects fetal outcome in diabetic pregnancies - and since no hydrolysis of unconjugated E3 is required, a more rapid tumaround for the test result is possible. |
| Highlight | Related products: Estriol antibodies; Estriol ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit; Signaling Transduction kit |
Images (1) Click the Picture to Zoom In
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ARG80842 Human Estriol (unconjugated) ELISA Kit example of standard curve image
ARG80842 Human Estriol (unconjugated) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG80842 Human Estriol (unconjugated) ELISA Kit User's manual |
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