ARG81470
Human Entactin ELISA Kit
Human Entactin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81470-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81470-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81470-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81470-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81470-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81470-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81470-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81470-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81470-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81470-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81470-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81470 Human Entactin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Entactin in serum and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Entactin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.8% Inter-Assay CV: 6.5% |
| Alternate Names | Entactin; NID-1; Nidogen-1; NID |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NID1 |
| Gene Full Name | nidogen 1 |
| Background | This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008] |
| Function | Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. [UniProt] |
| Highlight | Related products: Entactin antibodies; Entactin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81470 Human Entactin ELISA Kit standard curve image
ARG81470 Human Entactin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81470 Human Entactin ELISA Kit User's manual |
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