ARG81504
Human Elafin ELISA Kit
Human Elafin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81504-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81504-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81504-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81504-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81504-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81504-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81504-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81504-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81504-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81504-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81504-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81504 Human Elafin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Elafin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Elafin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7% Inter-Assay CV: 6.5% |
| Alternate Names | Protease inhibitor WAP3; cementoin; WFDC14; Skin-derived antileukoproteinase; WAP four-disulfide core domain protein 14; SKALP; Elafin; Elastase-specific inhibitor; WAP3; Peptidase inhibitor 3; ESI; PI-3 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PI3 |
| Gene Full Name | peptidase inhibitor 3, skin-derived |
| Background | This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. [provided by RefSeq, Oct 2014] |
| Function | Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. [UniProt] |
| Highlight | Related products: Elafin antibodies; Elafin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81504 Human Elafin ELISA Kit standard curve image
ARG81504 Human Elafin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81504 Human Elafin ELISA Kit User's manual |
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